| Title |
Emergent genetic oscillations in a synthetic microbial consortium
|
|---|---|
| Published in |
Science, August 2015
|
| DOI | 10.1126/science.aaa3794 |
| Pubmed ID | |
| Authors |
Ye Chen, Jae Kyoung Kim, Andrew J Hirning, Krešimir Josić, Matthew R Bennett |
| Abstract |
A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types-an "activator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types. |
X Demographics
The data shown below were collected from the profiles of 32 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 34% |
| Spain | 2 | 6% |
| Netherlands | 1 | 3% |
| Venezuela, Bolivarian Republic of | 1 | 3% |
| Korea, Republic of | 1 | 3% |
| United Kingdom | 1 | 3% |
| Unknown | 15 | 47% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 19 | 59% |
| Scientists | 12 | 38% |
| Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 559 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 13 | 2% |
| United Kingdom | 3 | <1% |
| France | 2 | <1% |
| Switzerland | 2 | <1% |
| Canada | 2 | <1% |
| Israel | 1 | <1% |
| Germany | 1 | <1% |
| Portugal | 1 | <1% |
| Singapore | 1 | <1% |
| Other | 7 | 1% |
| Unknown | 526 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 141 | 25% |
| Researcher | 103 | 18% |
| Student > Master | 61 | 11% |
| Student > Bachelor | 53 | 9% |
| Professor | 31 | 6% |
| Other | 80 | 14% |
| Unknown | 90 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 152 | 27% |
| Biochemistry, Genetics and Molecular Biology | 132 | 24% |
| Engineering | 49 | 9% |
| Immunology and Microbiology | 22 | 4% |
| Chemistry | 22 | 4% |
| Other | 72 | 13% |
| Unknown | 110 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 128. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#322,725
of 25,315,460 outputs
Outputs from Science
#8,329
of 80,802 outputs
Outputs of similar age
#3,858
of 274,919 outputs
Outputs of similar age from Science
#159
of 1,229 outputs
Altmetric has tracked 25,315,460 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 80,802 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,919 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 1,229 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.